RayCure SPE series provides high-quality SPE cartridges for various types of solid phase extraction, including manifold, vacuum as well as automated solid phase extraction equipment, well compatible with RayKol automated solid-phase extraction systems. RayCure SPE cartridges utilize high-quality sorbents to ensure the efficiency of SPE clean up, offers full range of SPE cartridges for various sample matices. Strict quality managment and control ensures high recovery rate of analytes and experiment reproducibility. RayKol provides effective, efficient and economial SPE cartridges with high performance.
The properties of solid phase packing in SPE cartridges should be match to target compounds.
Take the advantage of differences between analytes of interest and interferences
Consider nature of SPE sample matrices, characteristics of analytes and solubility of used solvents
Users can select RayCure SPE columns depending on properties of desired analytes and sample matrices as following selection guides.
|Target compounds||Properties of analytes||Corresponding SPE|
|Solubility||Polarity/Ionicity||SPE mechanism||Sorbent phase|
|Water soluble||Compounds with high/low polarity||Non-polar||C18|
|Ionic compounds||Anion exchange||MCX, WCX|
|Cation exchange||MAX, WAX|
|Soluble in organic solvents||Polar compounds||Normal phase bonding||PSA, NH2|
|Compounds with neutral polarity||Normal phase adsorption||Silica, Florisil|
|Non-polar compounds||Reversed phase||C18|
Based on nature of SPE sample matrices
|SPE sample matrices||Target compounds||Impurities||RayCure SPE column|
|Fruits, vegetables, fruit juice, vegetable juice, fruit wine, herbs||Multiple pesticide residues||Carbohydrates, pigments, organic acid, phenol||RayCure QuEChERS d-SPE kits|
|Weak polar pesticides||Carbohydrates, pigments, organic acid, phenol||RayCure HLB|
|Alkali pesticides||Carbohydrates, pigments, organic acid, phenol||RayCure MCX|
|Acidic pesticides||Carbohydrates, pigments, organic acid, phenol||RayCure MAX|
|Dairy products, blood, urine, animal tissues||Neutral, weak alkali, weak acidic drugs||Proteins, fats||RayCure HLB|
|Alkali drugs||Proteins, fats||RayCure MCX|
|Acidic drugs||Proteins, fats||RayCure MAX|
|Soil, wastewater||hydrophobic organic pollutants||Humic substances||RayCure HLB|
|Pesticide residues||Humic substances||RayCure Florisil|
|Oil and grease||Fat-soluble vitamins, phospholipids, aflatoxins||Fats||RayCure Silica|
SPE is a conventional and efficient extraction technique, it uses the affinity of solutes dissolved or suspended in mobile phase for stationary phase to separate desired compounds from a mixture. And there are two solid phase extraction mechanisms to obtain target analytes from complex samples, to retain desired compounds in stationary phase or retain impurities in solid phase. Depending on the difference of two mechanisms, SPE procedures could be a bit various.
When sample portion passes through SPE column, undesired impurities are discarded with mobile phase, target compounds are retained in stationary phase, then rinse stationary phase with eluents to collect target compounds. It is the most commonly used purification mechanism of solid phase extraction.
In the opposite, to retain impurities in stationary phase, target compounds and few impurities are collected in mobile phase when sample portion passes through SPE column. Rinse the stationary phase which impurities is retained with solvents to obtain rest of retained target compounds. This mechanism is mostly used to eliminate fat-soluble impurities of ion analysis and analysis of multiple pesticides in fruits and vegetables.