RayKol Automated Nucleic Acid Extraction Solution for Herbal Medicine: Boosting Molecular Identification

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The identification technology of traditional Chinese medicine can be divided into 4 main development stages: sensory evaluation, microscopic identification, physicochemical identification, and molecular identification. With the introduction of specific molecular identification methods for Buthus martensii, Dendrobium, Fritillaria, etc., into the "Chinese Pharmacopoeia," molecular identification has become a major trend in the work of Chinese medicine identification.

Nucleic acid extraction of Chinese medicinal materials is an indispensable step in the molecular identification of traditional Chinese medicine. The fully automated solution for nucleic acid extraction of traditional Chinese medicinal materials by RayKol can achieve automated extraction and purification of nucleic acids from Chinese medicinal materials, construct PCR reaction systems, and other steps, truly realizing "sample in - nucleic acid out," improving experimental efficiency and quality, and supporting molecular identification of Chinese medicinal materials.

Part 1: Solution

The fully automated solution for nucleic acid extraction of traditional Chinese medicinal materials by RayKol can meet the needs of high-throughput (1-96 samples/run) nucleic acid extraction. The independently developed nucleic acid extraction kits, combined with the Vitae100 nucleic acid extraction and purification instrument, can automatically complete processes such as sample nucleic acid extraction and system construction, achieving high-quality nucleic acids while reducing manual operation time and improving work efficiency.

RayKol fully automated solution for nucleic acid extraction of traditional Chinese medicinal materials

Part 2: Customer Case

Customers have used RayKol's nucleic acid extraction reagents and Vitae 100 nucleic acid extraction instruments to replace the original manual extraction method, freeing their hands and realizing automated nucleic acid extraction for leech samples.

Automated Experimental Process

The customer's laboratory previously used T brand column extraction kits for manual extraction of leech nucleic acids, which involved many and cumbersome steps. The fully automated solution for nucleic acid extraction of traditional Chinese medicinal materials by RayKol only requires the samples to be loaded, initiating the system's preset experimental process for nucleic acid extraction, truly realizing "sample in - nucleic acid out."

The Nucleic Acid Extraction Results Meet the Needs of Subsequent Experiments

In this case, the fully automated solution for nucleic acid extraction of traditional Chinese medicinal materials by RayKol extracted nucleic acid concentrations from different leech samples of about 180-500ng/μL, with some results even better than those obtained by manual extraction. The nucleic acid yield and quality met the needs of the customer's subsequent experiments.

Table 1: Nucleic acid extraction results for different types of leech samples using the RayKol solution

Summary

The RayKol nucleic acid extraction solution for traditional Chinese medicinal materials can truly achieve the fully automated process of "sample in - nucleic acid out,” with high yield and good quality of the extracted nucleic acids.